› 5enl

X-ray diffraction
2.2Å resolution

INHIBITION OF ENOLASE: THE CRYSTAL STRUCTURES OF ENOLASE-CA2+-PHOSPHOGLYCERATE AND ENOLASE-ZN2+-PHOSPHOGLYCOLATE COMPLEXES AT 2.2-ANGSTROMS RESOLUTION

Released:
DOI: 10.2210/pdb5enl/pdb
PDB entry 5enl coloured by chain, front view.
PDB entry 5enl coloured by chain, side view.
PDB entry 5enl coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Inhibition of enolase: the crystal structures of enolase-Ca2(+)- 2-phosphoglycerate and enolase-Zn2(+)-phosphoglycolate complexes at 2.2-A resolution.
Lebioda L, Stec B, Brewer JM, Tykarska E
Biochemistry 30 2823-7 (1991)
PMID: 2007121

Function and Biology Details

Reaction catalysed: 
2-phospho-D-glycerate = phosphoenolpyruvate + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133690 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Enolase 1 Chain: A
Molecule details ›
Chain: A
Length: 436 amino acids
Theoretical weight: 46.69 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided
UniProt:
  • Canonical: P00924 (Residues: 2-437; Coverage: 100%)
Gene names: ENO1, ENOA, G9160, HSP48, YGR254W
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CA 1 x CA
Ligand 2PG 1 x 2PG
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P42212
Unit cell:
a: 124.1Å b: 124.1Å c: 66.9Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.148 not available not available
Expression system: Not provided

Quick links

Citations

2 review citations

A structural role for arginine in proteins: multiple hydrogen bonds to backbone carbonyl oxygens.
Borders et al. (1994)
1 more

4 mentions without citation

The Catalytic Site Atlas: a resource of catalytic sites and residues identified in enzymes using structural data.
Porter et al. (2004)
3 more