› 5es1

X-ray diffraction
2.8Å resolution

CRYSTAL STRUCTURE OF MICROTUBULE AFFINITY-REGULATING KINASE 4 CATALYTIC DOMAIN IN COMPLEX WITH A PYRAZOLOPYRIMIDINE INHIBITOR

Released:
DOI: 10.2210/pdb5es1/pdb
PDB entry 5es1 coloured by chain, front view.
PDB entry 5es1 coloured by chain, side view.
PDB entry 5es1 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of microtubule affinity-regulating kinase 4 catalytic domain in complex with a pyrazolopyrimidine inhibitor.
Sack JS, Gao M, Kiefer SE, Myers JE, Newitt JA, Wu S, Yan C
Acta Crystallogr F Struct Biol Commun 72 129-34 (2016)
PMID: 26841763

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188605 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
MAP/microtubule affinity-regulating kinase 4 Chain: A
Molecule details ›
Chain: A
Length: 328 amino acids
Theoretical weight: 37.62 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q96L34 (Residues: 44-370; Coverage: 44%)
Gene names: KIAA1860, MARK4, MARKL1
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand 5RC 1 x 5RC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P62
Unit cell:
a: 111.478Å b: 111.478Å c: 69.775Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.243 0.239 0.311
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Regulation of Cell Polarity by PAR-1/MARK Kinase.
Wu et al. (2017)
1 more

20 mentions without citation

Rosmarinic Acid Exhibits Anticancer Effects via MARK4 Inhibition.
Anwar et al. (2020)
19 more