Function and Biology Details
Reactions catalysed:
SsDNA + n NTP = ssDNA/pppN(pN)(n-1) hybrid + (n-1) diphosphate
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
- DNA-directed DNA polymerase, family B
- DNA polymerase alpha, subunit B
- DNA primase large subunit, eukaryotic/archaeal
- DNA primase, large subunit, eukaryotic
- DNA primase, small subunit, eukaryotic/archaeal
- DNA polymerase alpha/delta/epsilon, subunit B
- DNA polymerase alpha, subunit B, N-terminal
- DNA polymerase alpha, subunit B, N-terminal domain superfamily
12 more domains
Structure analysis Details
Assembly composition:
hetero tetramer (preferred)
Assembly name:
DNA polymerase alpha:primase complex (preferred)
PDBe Complex ID:
PDB-CPX-140801 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
DNA primase small subunit
Molecule details ›
Chains: A, E
Length: 420 amino acids
Theoretical weight: 49.98 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
Sequence domains: DNA primase small subunit
Structure domains: DNA primase, PRIM domain
Length: 420 amino acids
Theoretical weight: 49.98 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
- Canonical:
P49642 (Residues: 1-420; Coverage: 100%)
Sequence domains: DNA primase small subunit
Structure domains: DNA primase, PRIM domain
DNA primase large subunit
Molecule details ›
Chains: B, F
Length: 509 amino acids
Theoretical weight: 58.89 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
Sequence domains: Eukaryotic and archaeal DNA primase, large subunit
Length: 509 amino acids
Theoretical weight: 58.89 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
- Canonical: P49643 (Residues: 1-509; Coverage: 100%)
Sequence domains: Eukaryotic and archaeal DNA primase, large subunit
DNA polymerase alpha catalytic subunit
Molecule details ›
Chains: C, G
Length: 1128 amino acids
Theoretical weight: 129.31 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
Sequence domains:
Length: 1128 amino acids
Theoretical weight: 129.31 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
- Canonical: P09884 (Residues: 335-1462; Coverage: 77%)
Sequence domains:
DNA polymerase alpha subunit B
Molecule details ›
Chains: D, H
Length: 597 amino acids
Theoretical weight: 65.88 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
Sequence domains:
Length: 597 amino acids
Theoretical weight: 65.88 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
- Canonical: Q14181 (Residues: 2-598; Coverage: 100%)
Sequence domains:
