› 5exu

X-ray diffraction
1.65Å resolution

Reversibly photoswitching protein Dathail, Ensemble refinement

Released:
DOI: 10.2210/pdb5exu/pdb
PDB entry 5exu coloured by chain, ensemble of 63 models, front view.
PDB entry 5exu coloured by chain, ensemble of 63 models, side view.
PDB entry 5exu coloured by chain, ensemble of 63 models, top view.
Source organism: synthetic construct
Primary publication:
Evolution and characterization of a new reversibly photoswitching chromogenic protein, Dathail.
Langan PS, Close DW, Coates L, Rocha RC, Ghosh K, Kiss C, Waldo G, Freyer J, Kovalevsky A, Bradbury AR
J Mol Biol 428 1776-89 (2016)
PMID: 27000644

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-165624 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Reversibly photoswitching protein Dathail Chain: A
Molecule details ›
Chain: A
Length: 229 amino acids
Theoretical weight: 25.9 KDa
Source organism: synthetic construct
Expression system: Escherichia coli BL21
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand CRQ 1 x CRQ

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P21212
Unit cell:
a: 76.25Å b: 81.18Å c: 39.47Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.171 0.168 0.21
Expression system: Escherichia coli BL21

Quick links

Citations

2 review citations

Identifying and Visualizing Macromolecular Flexibility in Structural Biology.
Palamini et al. (2016)
1 more