› 5f0k

X-ray diffraction
3.07Å resolution

Structure of VPS35 N terminal region

Released:
DOI: 10.2210/pdb5f0k/pdb
PDB entry 5f0k coloured by chain, front view.
PDB entry 5f0k coloured by chain, side view.
PDB entry 5f0k coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural Mechanism for Cargo Recognition by the Retromer Complex.
Lucas M, Gershlick DC, Vidaurrazaga A, Rojas AL, Bonifacino JS, Hierro A
Cell 167 1623-1635.e14 (2016)
PMID: 27889239

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188701 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Vacuolar protein sorting-associated protein 35 Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 462 amino acids
Theoretical weight: 53.29 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q96QK1 (Residues: 14-470; Coverage: 57%)
Gene names: MEM3, TCCCTA00141, VPS35
Sequence domains: Vacuolar protein sorting-associated protein 35

Ligands and Environments

4 bound ligands:
Ligand EDO 14 x EDO
Ligand GOL 9 x GOL
Ligand SO4 3 x SO4
Ligand PG4 1 x PG4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: C2
Unit cell:
a: 200.7Å b: 108.6Å c: 167.9Å
α: 90° β: 125.5° γ: 90°
R-values:
R R work R free
0.208 0.206 0.255
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

35 review citations

Endosomal receptor trafficking: Retromer and beyond.
Wang et al. (2018)
34 more

1 mention without citation

Structural Mechanism for Cargo Recognition by the Retromer Complex.
Lucas et al. (2016)