› 5f4t

X-ray diffraction
3.08Å resolution

Crystal structure of the human sperm Izumo1 residues 22-254

Released:
DOI: 10.2210/pdb5f4t/pdb
PDB entry 5f4t coloured by chain, front view.
PDB entry 5f4t coloured by chain, side view.
PDB entry 5f4t coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular architecture of the human sperm IZUMO1 and egg JUNO fertilization complex.
Aydin H, Sultana A, Li S, Thavalingam A, Lee JE
Nature 534 562-5 (2016)
PMID: 27309818

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184956 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Izumo sperm-egg fusion protein 1 Chain: A
Molecule details ›
Chain: A
Length: 239 amino acids
Theoretical weight: 27.18 KDa
Source organism: Homo sapiens
Expression system: Drosophila melanogaster
UniProt:
  • Canonical: Q8IYV9 (Residues: 22-254; Coverage: 71%)
Gene name: IZUMO1
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand NAG 1 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: R3
Unit cell:
a: 126.039Å b: 126.039Å c: 60.895Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.221 0.219 0.248
Expression system: Drosophila melanogaster

Quick links

Citations

21 review citations

The hallmarks of cell-cell fusion.
Hernández et al. (2017)
20 more

3 mentions without citation

The cell biology of fertilization: Gamete attachment and fusion.
Siu et al. (2021)
2 more