› 5fh9

X-ray diffraction
3.16Å resolution

Crystal structure of NFeoB from Escherichia coli BL21 in the apo state.

Released:
DOI: 10.2210/pdb5fh9/pdb
PDB entry 5fh9 coloured by chain, front view.
PDB entry 5fh9 coloured by chain, side view.
PDB entry 5fh9 coloured by chain, top view.
Source organism: Bacillus sp. SH51
Primary publication:
Studies on the X-Ray and Solution Structure of FeoB from Escherichia coli BL21.
Hagelueken G, Hoffmann J, Schubert E, Duthie FG, Florin N, Konrad L, Imhof D, Behrmann E, Morgner N, Schiemann O
Biophys. J. 110 2642-50 (2016)
PMID: 27332122

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125360 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ferrous iron transport protein B Chains: A, B
Molecule details ›
Chains: A, B
Length: 274 amino acids
Theoretical weight: 30.07 KDa
Source organism: Bacillus sp. SH51
Expression system: Escherichia coli
UniProt:
  • Canonical: J7QA66 (Residues: 1-274; Coverage: 35%)
Gene names: BANRA_04405, BGZ_04218, BK292_07590, BvCmsSIP010_02011, C0P57_000811, C1Q91_001707, C3F40_19215, CF22_001870, CG704_08025, CG831_001359, CV83915_02706, D4M65_08575, DIV22_04230, DRW19_02100, DS732_24845, DTL43_14135, E5H86_09570, E6D34_04350, EAI46_24105, EAN77_06985, EHD79_06380, EPS97_04485, ExPECSC038_01143, F9413_17310, FJQ40_03555, FZU14_04330, G4A38_17050, GAJ12_07085, GOP25_12605, GP965_18945, GQE86_01690, GQM04_09335, GUC01_14240, HHH44_000617, I6H02_12460, NCTC10429_00947, NCTC10865_00548, NCTC11181_02644, NCTC8960_02977, NCTC8985_04865, NEP60_16175, QDW62_01655, RZR61_07400, feoB
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: P63
Unit cell:
a: 166.675Å b: 166.675Å c: 65.581Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.209 0.208 0.228
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Why Nature Chose Potassium.
Danchin et al. (2019)
5 other articles

2 mentions without citation

Ins and Outs: Recent Advancements in Membrane Protein-Mediated Prokaryotic Ferrous Iron Transport.
Brown et al. (2021)
1 more