› 5fit

X-ray diffraction
2.3Å resolution

FHIT-SUBSTRATE ANALOG

Released:
DOI: 10.2210/pdb5fit/pdb
PDB entry 5fit coloured by chain, front view.
PDB entry 5fit coloured by chain, side view.
PDB entry 5fit coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure-based analysis of catalysis and substrate definition in the HIT protein family.
Lima CD, Klein MG, Hendrickson WA
Science 278 286-90 (1997)
PMID: 9323207

Function and Biology Details

Reactions catalysed: 
Adenylyl sulfate + NH(3) = adenosine 5'-phosphoramidate + sulfate
P(1)-P(3)-bis(5'-adenosyl) triphosphate + H(2)O = ADP + AMP
Adenylyl sulfate + H(2)O = AMP + sulfate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156029 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bis(5'-adenosyl)-triphosphatase Chain: A
Molecule details ›
Chain: A
Length: 147 amino acids
Theoretical weight: 16.98 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P49789 (Residues: 1-147; Coverage: 100%)
Gene name: FHIT
Sequence domains: HIT domain
Structure domains: HIT-like

Ligands and Environments

1 bound ligand:
Ligand AP2 1 x AP2
1 modified residue:
Ligand MSE 2 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P6122
Unit cell:
a: 50.64Å b: 50.64Å c: 267.52Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.226 0.226 0.258
Expression system: Escherichia coli

Quick links

Citations

29 review citations

Messenger RNA turnover in eukaryotes: pathways and enzymes.
Meyer et al. (2004)
28 more

2 mentions without citation

Selective prediction of interaction sites in protein structures with THEMATICS.
Wei et al. (2007)
1 more