› 5flz

Electron Microscopy
6.9Å resolution

Cryo-EM structure of gamma-TuSC oligomers in a closed conformation

Released:
DOI: 10.2210/pdb5flz/pdb
PDB entry 5flz coloured by chain, ensemble of 10 models, front view.
PDB entry 5flz coloured by chain, ensemble of 10 models, side view.
PDB entry 5flz coloured by chain, ensemble of 10 models, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structure of γ-tubulin small complex based on a cryo-EM map, chemical cross-links, and a remotely related structure.
Greenberg CH, Kollman J, Zelter A, Johnson R, MacCoss MJ, Davis TN, Agard DA, Sali A
J Struct Biol 194 303-10 (2016)
PMID: 26968363
Related structures: EMD-2799

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 42-mer (preferred)
PDBe Complex ID:
PDB-CPX-153757 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Spindle pole body component SPC97 Chain: A
Molecule details ›
Chain: A
Length: 823 amino acids
Theoretical weight: 96.94 KDa
Source organism: Saccharomyces cerevisiae
Expression system: unidentified baculovirus
UniProt:
  • Canonical: P38863 (Residues: 1-823; Coverage: 100%)
Gene names: SPC97, YHR172W
Sequence domains:
Spindle pole body component SPC98 Chain: B
Molecule details ›
Chain: B
Length: 846 amino acids
Theoretical weight: 98.34 KDa
Source organism: Saccharomyces cerevisiae
Expression system: unidentified baculovirus
UniProt:
  • Canonical: P53540 (Residues: 1-846; Coverage: 100%)
Gene names: N1222, N1879, SPC98, YNL126W
Sequence domains:
Tubulin gamma chain Chains: C, D
Molecule details ›
Chains: C, D
Length: 473 amino acids
Theoretical weight: 52.73 KDa
Source organism: Saccharomyces cerevisiae
Expression system: unidentified baculovirus
UniProt:
  • Canonical: P53378 (Residues: 1-473; Coverage: 100%)
Gene names: L8167.21, TUB4, YLR212C
Sequence domains:
SPINDLE POLE BODY COMPONENT 110 Chains: E, F
Molecule details ›
Chains: E, F
Length: 44 amino acids
Theoretical weight: 3.76 KDa
Source organism: Saccharomyces cerevisiae
Expression system: unidentified baculovirus

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 6.9Å
Relevant EMDB volumes: EMD-2799
Expression system: unidentified baculovirus

Quick links

Citations

2 review citations

Computational modeling of protein assemblies.
Soni et al. (2017)
1 more

7 mentions without citation

Structure of γ-tubulin small complex based on a cryo-EM map, chemical cross-links, and a remotely related structure.
Greenberg et al. (2016)
6 more