› 5fmv

X-ray diffraction
2.9Å resolution

Crystal structure of human CD45 extracellular region, domains d1-d4

Released:
DOI: 10.2210/pdb5fmv/pdb
PDB entry 5fmv coloured by chain, front view.
PDB entry 5fmv coloured by chain, side view.
PDB entry 5fmv coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Initiation of T cell signaling by CD45 segregation at 'close contacts'.
Chang VT, Fernandes RA, Ganzinger KA, Lee SF, Siebold C, McColl J, Jönsson P, Palayret M, Harlos K, Coles CH, Jones EY, Lui Y, Huang E, Gilbert RJC, Klenerman D, Aricescu AR, Davis SJ
Nat Immunol 17 574-582 (2016)
PMID: 26998761

Function and Biology Details

Reaction catalysed: 
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140183 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Receptor-type tyrosine-protein phosphatase C Chains: A, B
Molecule details ›
Chains: A, B
Length: 361 amino acids
Theoretical weight: 41.65 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P08575 (Residues: 225-573; Coverage: 27%)
Gene names: CD45, PTPRC
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand NAG 11 x NAG
Ligand SO4 5 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P21
Unit cell:
a: 94.55Å b: 59.41Å c: 100.42Å
α: 90° β: 111.66° γ: 90°
R-values:
R R work R free
0.227 0.226 0.249
Expression system: Cricetulus griseus

Quick links

Citations

40 review citations

TCR Signaling: Mechanisms of Initiation and Propagation.
Courtney et al. (2018)
39 more

9 mentions without citation

Initiation of T cell signaling by CD45 segregation at 'close contacts'.
Chang et al. (2016)
8 more