› 5fn2

Electron Microscopy
4.2Å resolution

Cryo-EM structure of gamma secretase in complex with a drug DAPT

Released:
DOI: 10.2210/pdb5fn2/pdb
PDB entry 5fn2 coloured by chain, front view.
PDB entry 5fn2 coloured by chain, side view.
PDB entry 5fn2 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Sampling the conformational space of the catalytic subunit of human γ-secretase.
Bai XC, Rajendra E, Yang G, Shi Y, Scheres SH
Elife 4 (2015)
PMID: 26623517
Related structures: EMD-3237

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156020 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Nicastrin Chain: A
Molecule details ›
Chain: A
Length: 709 amino acids
Theoretical weight: 78.48 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q92542 (Residues: 1-709; Coverage: 100%)
Gene names: KIAA0253, NCSTN, UNQ1874/PRO4317
Sequence domains:
Presenilin-1 Chain: B
Molecule details ›
Chain: B
Length: 467 amino acids
Theoretical weight: 52.65 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P49768 (Residues: 1-467; Coverage: 100%)
Gene names: AD3, PS1, PSEN1, PSNL1
Sequence domains: Presenilin
Gamma-secretase subunit APH-1A Chain: C
Molecule details ›
Chain: C
Length: 265 amino acids
Theoretical weight: 29.02 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q96BI3 (Residues: 1-265; Coverage: 100%)
Gene names: APH1A, CGI-78, PSF, UNQ579/PRO1141
Sequence domains: Aph-1 protein
Gamma-secretase subunit PEN-2 Chain: D
Molecule details ›
Chain: D
Length: 101 amino acids
Theoretical weight: 12.04 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9NZ42 (Residues: 1-101; Coverage: 100%)
Gene names: MDS033, PEN2, PSENEN
Sequence domains: Presenilin enhancer-2 subunit of gamma secretase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.2Å
Relevant EMDB volumes: EMD-3237
Expression system: Homo sapiens

Quick links

Citations

44 review citations

The Canonical Notch Signaling Pathway: Structural and Biochemical Insights into Shape, Sugar, and Force.
Kovall et al. (2017)
43 more

27 mentions without citation

Substrate-Enzyme Interactions in Intramembrane Proteolysis: γ-Secretase as the Prototype.
Liu et al. (2020)
26 more