› 5fn4

Electron Microscopy
4Å resolution

Cryo-EM structure of gamma secretase in class 2 of the apo- state ensemble

Released:
DOI: 10.2210/pdb5fn4/pdb
PDB entry 5fn4 coloured by chain, front view.
PDB entry 5fn4 coloured by chain, side view.
PDB entry 5fn4 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Sampling the conformational space of the catalytic subunit of human γ-secretase.
Bai XC, Rajendra E, Yang G, Shi Y, Scheres SH
Elife 4 (2015)
PMID: 26623517
Related structures: EMD-3239

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156019 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Nicastrin Chain: A
Molecule details ›
Chain: A
Length: 709 amino acids
Theoretical weight: 78.48 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q92542 (Residues: 1-709; Coverage: 100%)
Gene names: KIAA0253, NCSTN, UNQ1874/PRO4317
Sequence domains:
Presenilin-1 Chain: B
Molecule details ›
Chain: B
Length: 467 amino acids
Theoretical weight: 52.71 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P49768 (Residues: 1-467; Coverage: 100%)
Gene names: AD3, PS1, PSEN1, PSNL1
Sequence domains: Presenilin
Gamma-secretase subunit APH-1A Chain: C
Molecule details ›
Chain: C
Length: 265 amino acids
Theoretical weight: 29.02 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q96BI3 (Residues: 1-265; Coverage: 100%)
Gene names: APH1A, CGI-78, PSF, UNQ579/PRO1141
Sequence domains: Aph-1 protein
Gamma-secretase subunit PEN-2 Chain: D
Molecule details ›
Chain: D
Length: 101 amino acids
Theoretical weight: 12.04 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9NZ42 (Residues: 1-101; Coverage: 100%)
Gene names: MDS033, PEN2, PSENEN
Sequence domains: Presenilin enhancer-2 subunit of gamma secretase
POLY ALA CHAIN Chain: G
Molecule details ›
Chain: G
Length: 25 amino acids
Theoretical weight: 1.79 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4Å
Relevant EMDB volumes: EMD-3239
Expression system: Homo sapiens

Quick links

Citations

43 review citations

The Canonical Notch Signaling Pathway: Structural and Biochemical Insights into Shape, Sugar, and Force.
Kovall et al. (2017)
42 more

11 mentions without citation

Identifying and Visualizing Macromolecular Flexibility in Structural Biology.
Palamini et al. (2016)
10 more