› 5fs9

X-ray diffraction
1.75Å resolution

Crystal structure of the G338E mutant of human apoptosis inducing factor

Released:
DOI: 10.2210/pdb5fs9/pdb
PDB entry 5fs9 coloured by chain, front view.
PDB entry 5fs9 coloured by chain, side view.
PDB entry 5fs9 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure/Function Relations in AIFM1 Variants Associated with Neurodegenerative Disorders.
Sevrioukova IF
J Mol Biol 428 3650-65 (2016)
PMID: 27178839

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132061 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Apoptosis-inducing factor 1, mitochondrial Chains: A, B
Molecule details ›
Chains: A, B
Length: 515 amino acids
Theoretical weight: 56.31 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O95831 (Residues: 103-613; Coverage: 83%)
Gene names: AIF, AIFM1, PDCD8
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand FAD 2 x FAD
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P212121
Unit cell:
a: 90.619Å b: 114.93Å c: 121.77Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.175 0.2
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

10 review citations

Apoptosis-Inducing Factor (AIF) in Physiology and Disease: The Tale of a Repented Natural Born Killer.
Bano et al. (2018)
9 more

1 mention without citation

Autophagy and Apoptosis Specific Knowledgebases-guided Systems Pharmacology Drug Research.
Fan et al. (2019)