› 5fuc

X-ray diffraction
2.7Å resolution

Biophysical and cellular characterisation of a junctional epitope antibody that locks IL-6 and gp80 together in a stable complex: implications for new therapeutic strategies

Released:
DOI: 10.2210/pdb5fuc/pdb
PDB entry 5fuc coloured by chain, front view.
PDB entry 5fuc coloured by chain, side view.
PDB entry 5fuc coloured by chain, top view.
Source organisms:
Primary publication:
Discovery of a junctional epitope antibody that stabilizes IL-6 and gp80 protein:protein interaction and modulates its downstream signaling.
Adams R, Burnley RJ, Valenzano CR, Qureshi O, Doyle C, Lumb S, Del Carmen Lopez M, Griffin R, McMillan D, Taylor RD, Meier C, Mori P, Griffin LM, Wernery U, Kinne J, Rapecki S, Baker TS, Lawson AD, Wright M, Ettorre A
Sci Rep 7 37716 (2017)
PMID: 28134246

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-209240 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Interleukin-6 Chains: A, B
Molecule details ›
Chains: A, B
Length: 166 amino acids
Theoretical weight: 18.98 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P05231 (Residues: 49-212; Coverage: 90%)
Gene names: IFNB2, IL6
Sequence domains: Interleukin-6/G-CSF/MGF family
Structure domains: Growth Hormone; Chain: A;
Soluble interleukin-6 receptor subunit alpha Chains: C, D
Molecule details ›
Chains: C, D
Length: 231 amino acids
Theoretical weight: 25.97 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P08887 (Residues: 111-322; Coverage: 47%)
Gene name: IL6R
Sequence domains: Interleukin-6 receptor alpha chain, binding
Structure domains: Immunoglobulins
VHH6 Chains: E, V
Molecule details ›
Chains: E, V
Length: 132 amino acids
Theoretical weight: 14.68 KDa
Source organism: Camelus dromedarius
Expression system: Homo sapiens
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand NAG 4 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: C2
Unit cell:
a: 249.03Å b: 67.8Å c: 78.16Å
α: 90° β: 104.53° γ: 90°
R-values:
R R work R free
0.231 0.231 0.287
Expression systems:
  • Escherichia coli
  • Homo sapiens

Quick links

Citations

6 review citations

Recommendations for performing, interpreting and reporting hydrogen deuterium exchange mass spectrometry (HDX-MS) experiments.
Masson et al. (2019)
5 more

21 mentions without citation

Discovery of a junctional epitope antibody that stabilizes IL-6 and gp80 protein:protein interaction and modulates its downstream signaling.
Adams et al. (2017)
20 more