› 5g25

X-ray diffraction
2.3Å resolution

Type IV-like pilin TTHA1218 from Thermus thermophilus

Released:
DOI: 10.2210/pdb5g25/pdb
PDB entry 5g25 coloured by chain, front view.
PDB entry 5g25 coloured by chain, side view.
PDB entry 5g25 coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Primary publication:
Structures of type IV pilins from Thermus thermophilus demonstrate similarities with type II secretion system pseudopilins.
Karuppiah V, Thistlethwaite A, Derrick JP
J Struct Biol 196 375-384 (2016)
PMID: 27612581

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177987 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable prepilin-like protein Chain: A
Molecule details ›
Chain: A
Length: 97 amino acids
Theoretical weight: 10.71 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5SIZ6 (Residues: 36-123; Coverage: 72%)
Gene name: TTHA1218

Ligands and Environments

1 bound ligand:
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02, DIAMOND BEAMLINE I04
Spacegroup: F4132
Unit cell:
a: 156.59Å b: 156.59Å c: 156.59Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.208 0.237
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Structure and function of minor pilins of type IV pili.
Jacobsen et al. (2020)
1 more

1 mention without citation

Structures of type IV pilins from Thermus thermophilus demonstrate similarities with type II secretion system pseudopilins.
Karuppiah et al. (2016)