5gao

Electron Microscopy
3.6Å resolution

Head region of the yeast spliceosomal U4/U6.U5 tri-snRNP

Released:
Source organism: Saccharomyces cerevisiae
Related structures: EMD-8013

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero undecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152415 (preferred)
Entry contents:
10 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (11 distinct):
Small nuclear ribonucleoprotein-associated protein B Chain: k
Molecule details ›
Chain: k
Length: 196 amino acids
Theoretical weight: 22.43 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P40018 (Residues: 1-196; Coverage: 100%)
Gene names: SMB1, YER029C
Sequence domains: LSM domain
Small nuclear ribonucleoprotein Sm D1 Chain: l
Molecule details ›
Chain: l
Length: 146 amino acids
Theoretical weight: 16.3 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q02260 (Residues: 1-146; Coverage: 100%)
Gene names: SMD1, YGR074W
Sequence domains: LSM domain
Structure domains: SH3 type barrels.
Small nuclear ribonucleoprotein Sm D2 Chain: m
Molecule details ›
Chain: m
Length: 110 amino acids
Theoretical weight: 12.88 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q06217 (Residues: 1-110; Coverage: 100%)
Gene names: L9328.5, SMD2, YLR275W
Sequence domains: LSM domain
Structure domains: SH3 type barrels.
Small nuclear ribonucleoprotein Sm D3 Chain: n
Molecule details ›
Chain: n
Length: 101 amino acids
Theoretical weight: 11.24 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P43321 (Residues: 1-101; Coverage: 100%)
Gene names: L9634.6, SMD3, YLR147C
Sequence domains: LSM domain
Structure domains: SH3 type barrels.
Small nuclear ribonucleoprotein E Chain: p
Molecule details ›
Chain: p
Length: 94 amino acids
Theoretical weight: 10.39 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q12330 (Residues: 1-94; Coverage: 100%)
Gene names: SME1, YOR159C
Sequence domains: LSM domain
Structure domains: SH3 type barrels.
Small nuclear ribonucleoprotein F Chain: q
Molecule details ›
Chain: q
Length: 86 amino acids
Theoretical weight: 9.67 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P54999 (Residues: 1-86; Coverage: 100%)
Gene names: P9705.4, SMX3, YPR182W
Sequence domains: LSM domain
Structure domains: SH3 type barrels.
Small nuclear ribonucleoprotein G Chain: r
Molecule details ›
Chain: r
Length: 77 amino acids
Theoretical weight: 8.49 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P40204 (Residues: 1-77; Coverage: 100%)
Gene names: SMX2, SNP2, YFL017Bw, YFL017W-A
Sequence domains: LSM domain
Structure domains: SH3 type barrels.
66 kDa U4/U6.U5 small nuclear ribonucleoprotein component Chain: E
Molecule details ›
Chain: E
Length: 338 amino acids
Theoretical weight: 25.08 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q12420 (Residues: 540-560; Coverage: 4%)
Gene names: 05667, SNU66, YOR308C
Pre-mRNA-splicing factor 8 Chain: A
Molecule details ›
Chain: A
Length: 267 amino acids
Theoretical weight: 30.23 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P33334 (Residues: 2147-2413; Coverage: 11%)
Gene names: DBF3, DNA39, PRP8, RNA8, SLT21, USA2, YHR165C
Sequence domains: PROCT (NUC072) domain
Structure domains: Cytidine Deaminase, domain 2
Saccharomyces cerevisiae strain UOA_M2 chromosome 5 sequence Chain: V
Molecule details ›
Chain: V
Length: 96 nucleotides
Theoretical weight: 30.62 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.6Å
Relevant EMDB volumes: EMD-8013