5gap

Electron Microscopy
3.6Å resolution

Body region of the U4/U6.U5 tri-snRNP

Released:
Source organism: Saccharomyces cerevisiae
Related structures: EMD-8014

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
PDBe Complex ID:
PDB-CPX-148703 (preferred)
Entry contents:
9 distinct polypeptide molecules
3 distinct RNA molecules
Macromolecules (12 distinct):
unknown protein Chain: x
Molecule details ›
Chain: x
Length: 82 amino acids
Theoretical weight: 7 KDa
Source organism: Saccharomyces cerevisiae
Pre-mRNA-splicing factor 8 Chain: A
U4/U6 small nuclear ribonucleoprotein PRP4 Chain: H
Molecule details ›
Chain: H
Length: 465 amino acids
Theoretical weight: 52.51 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P20053 (Residues: 1-465; Coverage: 100%)
Gene names: P9705.6, PRP4, RNA4, YPR178W
Sequence domains: WD domain, G-beta repeat
Pre-mRNA-splicing factor 6 Chain: J
Molecule details ›
Chain: J
Length: 899 amino acids
Theoretical weight: 104.37 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P19735 (Residues: 1-899; Coverage: 100%)
Gene names: PRP6, RNA6, YBR0508, YBR055C
Sequence domains: PRP1 splicing factor, N-terminal
Spliceosomal protein DIB1 Chain: D
Molecule details ›
Chain: D
Length: 143 amino acids
Theoretical weight: 16.8 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q06819 (Residues: 1-143; Coverage: 100%)
Gene names: CDH1, DIB1, P9513.12, SNU16, YPR082C
Sequence domains: Mitosis protein DIM1
Structure domains: Glutaredoxin
Pre-mRNA-processing factor 31 Chain: F
Molecule details ›
Chain: F
Length: 494 amino acids
Theoretical weight: 56.38 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P49704 (Residues: 1-494; Coverage: 100%)
Gene names: PRP31, YGR091W
Sequence domains:
U4/U6 small nuclear ribonucleoprotein PRP3 Chain: G
Molecule details ›
Chain: G
Length: 469 amino acids
Theoretical weight: 55.97 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q03338 (Residues: 1-469; Coverage: 100%)
Gene names: D8035.16, PRP3, RNA3, YDR473C
Sequence domains:
13 kDa ribonucleoprotein-associated protein Chain: K
Molecule details ›
Chain: K
Length: 126 amino acids
Theoretical weight: 13.58 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P39990 (Residues: 1-126; Coverage: 100%)
Gene names: SNU13, YEL026W
Sequence domains: Ribosomal protein L7Ae/L30e/S12e/Gadd45 family
Structure domains: 60s Ribosomal Protein L30; Chain: A;
Pre-mRNA-splicing helicase BRR2 Chain: B
Molecule details ›
Chain: B
Length: 2163 amino acids
Theoretical weight: 246.47 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P32639 (Residues: 1-2163; Coverage: 100%)
Gene names: BRR2, RSS1, SNU246, SYGP-ORF66, YER172C
Sequence domains:
U4 snRNA, 5' region, nucleotides 1-67 Chain: V
Molecule details ›
Chain: V
Length: 67 nucleotides
Theoretical weight: 21.53 KDa
Sequence domains: U4 spliceosomal RNA
U6 snRNA Chain: W
Molecule details ›
Chain: W
Length: 112 nucleotides
Theoretical weight: 35.88 KDa
Sequence domains: U6 spliceosomal RNA
U5 snRNA Chain: U
Molecule details ›
Chain: U
Length: 214 nucleotides
Theoretical weight: 68.64 KDa
Sequence domains: U5 spliceosomal RNA

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.6Å
Relevant EMDB volumes: EMD-8014