› 5gjr

Electron Microscopy
3.5Å resolution

An atomic structure of the human 26S proteasome

Released:
DOI: 10.2210/pdb5gjr/pdb
PDB entry 5gjr coloured by chain, front view.
PDB entry 5gjr coloured by chain, side view.
PDB entry 5gjr coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
An atomic structure of the human 26S proteasome.
Huang X, Luan B, Wu J, Shi Y
Nat Struct Mol Biol 23 778-85 (2016)
PMID: 27428775
Related structures: EMD-9512

Function and Biology Details

Reaction catalysed: 
Cleavage of peptide bonds with very broad specificity.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 64-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126191 (preferred)
Entry contents:
32 distinct polypeptide molecules
Macromolecules (32 distinct):
26S proteasome regulatory subunit 4 Chains: I, w
Molecule details ›
Chains: I, w
Length: 440 amino acids
Theoretical weight: 49.26 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P62191 (Residues: 1-440; Coverage: 100%)
Gene name: PSMC1
Sequence domains:
26S proteasome regulatory subunit 7 Chains: H, v
Molecule details ›
Chains: H, v
Length: 433 amino acids
Theoretical weight: 48.7 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P35998 (Residues: 1-433; Coverage: 100%)
Gene names: MSS1, PSMC2
Sequence domains:
26S proteasome regulatory subunit 10B Chains: L, z
Molecule details ›
Chains: L, z
Length: 389 amino acids
Theoretical weight: 44.24 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P62333 (Residues: 1-389; Coverage: 100%)
Gene names: PSMC6, SUG2
Sequence domains:
26S proteasome regulatory subunit 6A Chains: 0, M
Molecule details ›
Chains: 0, M
Length: 439 amino acids
Theoretical weight: 49.27 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P17980 (Residues: 1-439; Coverage: 100%)
Gene names: PSMC3, TBP1
Sequence domains:
26S proteasome regulatory subunit 8 Chains: J, x
Molecule details ›
Chains: J, x
Length: 406 amino acids
Theoretical weight: 45.69 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P62195 (Residues: 1-406; Coverage: 100%)
Gene names: PSMC5, SUG1
Sequence domains:
26S proteasome regulatory subunit 6B Chains: K, y
Molecule details ›
Chains: K, y
Length: 418 amino acids
Theoretical weight: 47.43 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P43686 (Residues: 1-418; Coverage: 100%)
Gene names: MIP224, PSMC4, TBP7
Sequence domains:
26S proteasome non-ATPase regulatory subunit 1 Chains: 1, N
Molecule details ›
Chains: 1, N
Length: 953 amino acids
Theoretical weight: 105.96 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q99460 (Residues: 1-953; Coverage: 100%)
Gene name: PSMD1
Sequence domains:
26S proteasome non-ATPase regulatory subunit 13 Chains: 2, O
Molecule details ›
Chains: 2, O
Length: 376 amino acids
Theoretical weight: 43 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q9UNM6 (Residues: 1-376; Coverage: 100%)
Gene name: PSMD13
Sequence domains: PCI domain
26S proteasome non-ATPase regulatory subunit 12 Chains: 3, P
Molecule details ›
Chains: 3, P
Length: 456 amino acids
Theoretical weight: 52.98 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: O00232 (Residues: 1-456; Coverage: 100%)
Gene name: PSMD12
Sequence domains:
26S proteasome non-ATPase regulatory subunit 11 Chains: 4, Q
Molecule details ›
Chains: 4, Q
Length: 422 amino acids
Theoretical weight: 47.53 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: O00231 (Residues: 1-422; Coverage: 100%)
Gene name: PSMD11
Sequence domains:
26S proteasome non-ATPase regulatory subunit 6 Chains: 5, R
Molecule details ›
Chains: 5, R
Length: 389 amino acids
Theoretical weight: 45.59 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q15008 (Residues: 1-389; Coverage: 100%)
Gene names: KIAA0107, PFAAP4, PSMD6
Sequence domains:
26S proteasome non-ATPase regulatory subunit 3 Chains: 6, S
Molecule details ›
Chains: 6, S
Length: 525 amino acids
Theoretical weight: 60.07 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: O43242 (Residues: 1-525; Coverage: 98%)
Gene name: PSMD3
Sequence domains:
26S proteasome non-ATPase regulatory subunit 8 Chains: 7, T
Molecule details ›
Chains: 7, T
Length: 350 amino acids
Theoretical weight: 39.67 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P48556 (Residues: 1-350; Coverage: 100%)
Gene name: PSMD8
Sequence domains: CSN8/PSMD8/EIF3K family
26S proteasome non-ATPase regulatory subunit 7 Chains: 8, U
Molecule details ›
Chains: 8, U
Length: 324 amino acids
Theoretical weight: 37.09 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P51665 (Residues: 1-324; Coverage: 100%)
Gene names: MOV34L, PSMD7
Sequence domains:
26S proteasome non-ATPase regulatory subunit 14 Chains: 9, V
Molecule details ›
Chains: 9, V
Length: 310 amino acids
Theoretical weight: 34.62 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: O00487 (Residues: 1-310; Coverage: 100%)
Gene names: POH1, PSMD14
Sequence domains: JAB1/Mov34/MPN/PAD-1 ubiquitin protease
26S proteasome non-ATPase regulatory subunit 4 Chains: AA, W
Molecule details ›
Chains: AA, W
Length: 377 amino acids
Theoretical weight: 40.78 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P55036 (Residues: 1-377; Coverage: 100%)
Gene names: MCB1, PSMD4
Sequence domains:
26S proteasome complex subunit SEM1 Chains: AB, Y
Molecule details ›
Chains: AB, Y
Length: 70 amino acids
Theoretical weight: 8.28 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P60896 (Residues: 1-70; Coverage: 100%)
Gene names: C7orf76, DSS1, SEM1, SHFDG1, SHFM1
Sequence domains: DSS1/SEM1 family
26S proteasome non-ATPase regulatory subunit 2 Chains: AC, Z
Molecule details ›
Chains: AC, Z
Length: 908 amino acids
Theoretical weight: 100.31 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q13200 (Residues: 1-908; Coverage: 100%)
Gene names: PSMD2, TRAP2
Sequence domains:
Proteasome subunit alpha type-6 Chains: B, h
Molecule details ›
Chains: B, h
Length: 246 amino acids
Theoretical weight: 27.43 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P60900 (Residues: 1-246; Coverage: 100%)
Gene names: PROS27, PSMA6
Sequence domains:
Proteasome subunit alpha type-2 Chains: C, i
Molecule details ›
Chains: C, i
Length: 234 amino acids
Theoretical weight: 25.93 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P25787 (Residues: 1-234; Coverage: 100%)
Gene names: HC3, PSC3, PSMA2
Sequence domains:
Proteasome subunit alpha type-4 Chains: D, j
Molecule details ›
Chains: D, j
Length: 261 amino acids
Theoretical weight: 29.53 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P25789 (Residues: 1-261; Coverage: 100%)
Gene names: HC9, PSC9, PSMA4
Sequence domains:
Proteasome subunit alpha type-7 Chains: E, k
Molecule details ›
Chains: E, k
Length: 248 amino acids
Theoretical weight: 27.93 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: O14818 (Residues: 1-248; Coverage: 100%)
Gene names: HSPC, PSMA7
Sequence domains:
Proteasome subunit alpha type-5 Chains: F, l
Molecule details ›
Chains: F, l
Length: 241 amino acids
Theoretical weight: 26.44 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P28066 (Residues: 1-241; Coverage: 100%)
Gene name: PSMA5
Sequence domains:
Proteasome subunit alpha type-1 Chains: G, m
Molecule details ›
Chains: G, m
Length: 263 amino acids
Theoretical weight: 29.6 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P25786 (Residues: 1-263; Coverage: 100%)
Gene names: HC2, NU, PROS30, PSC2, PSMA1
Sequence domains:
Proteasome subunit alpha type-3 Chains: X, n
Molecule details ›
Chains: X, n
Length: 255 amino acids
Theoretical weight: 28.47 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P25788 (Residues: 1-255; Coverage: 100%)
Gene names: HC8, PSC8, PSMA3
Sequence domains:
Proteasome subunit beta type-6 Chains: a, o
Molecule details ›
Chains: a, o
Length: 239 amino acids
Theoretical weight: 25.38 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P28072 (Residues: 1-239; Coverage: 100%)
Gene names: LMPY, PSMB6, Y
Sequence domains: Proteasome subunit
Proteasome subunit beta type-7 Chains: b, p
Molecule details ›
Chains: b, p
Length: 277 amino acids
Theoretical weight: 30 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q99436 (Residues: 1-277; Coverage: 100%)
Gene names: PSMB7, Z
Sequence domains:
Proteasome subunit beta type-3 Chains: c, q
Molecule details ›
Chains: c, q
Length: 205 amino acids
Theoretical weight: 22.97 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P49720 (Residues: 1-205; Coverage: 100%)
Gene name: PSMB3
Sequence domains: Proteasome subunit
Proteasome subunit beta type-2 Chains: d, r
Molecule details ›
Chains: d, r
Length: 201 amino acids
Theoretical weight: 22.86 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P49721 (Residues: 1-201; Coverage: 100%)
Gene name: PSMB2
Sequence domains: Proteasome subunit
Proteasome subunit beta type-5 Chains: e, s
Molecule details ›
Chains: e, s
Length: 263 amino acids
Theoretical weight: 28.51 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P28074 (Residues: 1-263; Coverage: 100%)
Gene names: LMPX, MB1, PSMB5, X
Sequence domains: Proteasome subunit
Proteasome subunit beta type-1 Chains: f, t
Molecule details ›
Chains: f, t
Length: 241 amino acids
Theoretical weight: 26.52 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P20618 (Residues: 1-241; Coverage: 100%)
Gene names: PSC5, PSMB1
Sequence domains: Proteasome subunit
Proteasome subunit beta type-4 Chains: g, u
Molecule details ›
Chains: g, u
Length: 264 amino acids
Theoretical weight: 29.23 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P28070 (Residues: 1-264; Coverage: 100%)
Gene names: PROS26, PSMB4
Sequence domains: Proteasome subunit

Ligands and Environments

1 bound ligand:
Ligand ADP 12 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.5Å
Relevant EMDB volumes: EMD-9512

Quick links

Citations

36 review citations

The Logic of the 26S Proteasome.
Collins et al. (2017)
35 more

27 mentions without citation

A Practical Review of Proteasome Pharmacology.
Thibaudeau et al. (2019)
26 more