› 5gwd

X-ray diffraction
1.89Å resolution

Structure of Myroilysin

Released:
DOI: 10.2210/pdb5gwd/pdb
PDB entry 5gwd coloured by chain, front view.
PDB entry 5gwd coloured by chain, side view.
PDB entry 5gwd coloured by chain, top view.
Source organism: Myroides sp. CSLB8
Primary publication:
Myroilysin Is a New Bacterial Member of the M12A Family of Metzincin Metallopeptidases and Is Activated by a Cysteine Switch Mechanism.
Xu D, Zhou J, Lou X, He J, Ran T, Wang W
J Biol Chem 292 5195-5206 (2017)
PMID: 28188295

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102563 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peptidase metallopeptidase domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 252 amino acids
Theoretical weight: 28.4 KDa
Source organism: Myroides sp. CSLB8
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0P0DZ84 (Residues: 31-273; Coverage: 89%)
Sequence domains: Astacin (Peptidase family M12A)

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
1 modified residue:
Ligand MLY 10 x MLY

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P21
Unit cell:
a: 72.223Å b: 35.486Å c: 93.757Å
α: 90° β: 101.41° γ: 90°
R-values:
R R work R free
0.184 0.182 0.224
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Protease-triggered bioresponsive drug delivery for the targeted theranostics of malignancy.
Li et al. (2021)
1 other article

4 mentions without citation

Myroilysin Is a New Bacterial Member of the M12A Family of Metzincin Metallopeptidases and Is Activated by a Cysteine Switch Mechanism.
Xu et al. (2017)
3 more