5h64

Electron Microscopy
4.4Å resolution

Cryo-EM structure of mTORC1

Released:
Source organism: Homo sapiens
Primary publication:
4.4 Å Resolution Cryo-EM structure of human mTOR Complex 1.
Protein Cell 7 878-887 (2016)
PMID: 27909983
Related structures: EMD-6668

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154637 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Serine/threonine-protein kinase mTOR Chains: A, a
Molecule details ›
Chains: A, a
Length: 2549 amino acids
Theoretical weight: 289.26 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P42345 (Residues: 1-2549; Coverage: 100%)
Gene names: FRAP, FRAP1, FRAP2, MTOR, RAFT1, RAPT1
Sequence domains:
Regulatory-associated protein of mTOR Chains: B, b
Molecule details ›
Chains: B, b
Length: 1335 amino acids
Theoretical weight: 149.2 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q8N122 (Residues: 1-1335; Coverage: 100%)
Gene names: KIAA1303, RAPTOR, RPTOR
Sequence domains:
Target of rapamycin complex subunit LST8 Chains: C, c
Molecule details ›
Chains: C, c
Length: 326 amino acids
Theoretical weight: 35.91 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9BVC4 (Residues: 1-326; Coverage: 100%)
Gene names: GBL, LST8, MLST8
Sequence domains: WD domain, G-beta repeat

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.4Å
Relevant EMDB volumes: EMD-6668
Expression system: Homo sapiens