› 5ham

X-ray diffraction
2.4Å resolution

Structure of Rickettsia bellii effector protein RickCE

Released:
DOI: 10.2210/pdb5ham/pdb
PDB entry 5ham coloured by chain, front view.
PDB entry 5ham coloured by chain, side view.
PDB entry 5ham coloured by chain, top view.
Source organism: Rickettsia bellii
Primary publication:
The Molecular Basis for Ubiquitin and Ubiquitin-like Specificities in Bacterial Effector Proteases.
Pruneda JN, Durkin CH, Geurink PP, Ovaa H, Santhanam B, Holden DW, Komander D
Mol Cell 63 261-276 (2016)
PMID: 27425412

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173051 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquitin-like protease family profile domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 274 amino acids
Theoretical weight: 31.1 KDa
Source organism: Rickettsia bellii
Expression system: Escherichia coli
UniProt:
  • Canonical: Q1RK35 (Residues: 420-691; Coverage: 38%)
Gene name: RBE_0198

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: R3
Unit cell:
a: 173.496Å b: 173.496Å c: 55.516Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.167 0.164 0.217
Expression system: Escherichia coli

Quick links

Citations

29 review citations

Mechanisms of Deubiquitinase Specificity and Regulation.
Mevissen et al. (2017)
28 more

2 mentions without citation

A deubiquitylase with an unusually high-affinity ubiquitin-binding domain from the scrub typhus pathogen Orientia tsutsugamushi.
Berk et al. (2020)
1 more