› 5han

X-ray diffraction
2.04Å resolution

Structure function studies of R. palustris RubisCO (S59F mutant; CABP-bound)

Released:
DOI: 10.2210/pdb5han/pdb
PDB entry 5han coloured by chain, front view.
PDB entry 5han coloured by chain, side view.
PDB entry 5han coloured by chain, top view.
Source organism: Rhodopseudomonas palustris
Entry authors: Arbing MA, Leong JG, Varaljay VA, Satagopan S, North JA, Tabita FR

Function and Biology Details

Reaction catalysed: 
2 3-phospho-D-glycerate + 2 H(+) = D-ribulose 1,5-bisphosphate + CO(2) + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179932 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribulose bisphosphate carboxylase Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 481 amino acids
Theoretical weight: 52.82 KDa
Source organism: Rhodopseudomonas palustris
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6N0W9 (Residues: 1-461; Coverage: 100%)
Gene names: RPA4641, cbbM
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CAP 12 x CAP
Ligand MG 12 x MG
1 modified residue:
Ligand KCX 12 x KCX

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P1
Unit cell:
a: 75.88Å b: 110.37Å c: 167.36Å
α: 89.87° β: 101.7° γ: 105°
R-values:
R R work R free
0.201 0.197 0.237
Expression system: Escherichia coli BL21(DE3)

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