› 5hbt

X-ray diffraction
2.61Å resolution

Complex structure of Fab35 and human nAChR alpha1

Released:
DOI: 10.2210/pdb5hbt/pdb
PDB entry 5hbt coloured by chain, front view.
PDB entry 5hbt coloured by chain, side view.
PDB entry 5hbt coloured by chain, top view.
Source organisms:
Primary publication:
Structural insights into the molecular mechanisms of myasthenia gravis and their therapeutic implications.
Noridomi K, Watanabe G, Hansen MN, Han GW, Chen L
Elife 6 (2017)
PMID: 28440223

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-208778 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (5 distinct):
Acetylcholine receptor subunit alpha Chain: B
Molecule details ›
Chain: B
Length: 212 amino acids
Theoretical weight: 24.63 KDa
Source organism: Homo sapiens
Expression system: Pichia
UniProt:
  • Canonical: P02708 (Residues: 21-231; Coverage: 48%)
Gene names: ACHRA, CHNRA, CHRNA1
Sequence domains: Neurotransmitter-gated ion-channel ligand binding domain
Structure domains: Neurotransmitter-gated ion-channel ligand-binding domain
Fab35, Light Chain Chain: C
Molecule details ›
Chain: C
Length: 213 amino acids
Theoretical weight: 23.39 KDa
Source organism: Rattus norvegicus
Structure domains: Immunoglobulins
Fab35, Heavy Chain Chain: D
Molecule details ›
Chain: D
Length: 219 amino acids
Theoretical weight: 23.88 KDa
Source organism: Rattus norvegicus
Structure domains: Immunoglobulins
Alpha-bungarotoxin isoform V31 Chain: A
Molecule details ›
Chain: A
Length: 74 amino acids
Theoretical weight: 8.03 KDa
Source organism: Bungarus multicinctus
UniProt:
  • Canonical: P60616 (Residues: 22-95; Coverage: 100%)
Structure domains: CD59

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: C2
Unit cell:
a: 160.006Å b: 42.143Å c: 136.488Å
α: 90° β: 117.05° γ: 90°
R-values:
R R work R free
0.21 0.207 0.26
Expression system: Pichia

Quick links

Citations

4 review citations

Progress in nicotinic receptor structural biology.
Gharpure et al. (2020)
3 more

2 mentions without citation

Structural insights into the molecular mechanisms of myasthenia gravis and their therapeutic implications.
Noridomi et al. (2017)
1 more