› 5hdn

X-ray diffraction
1.68Å resolution

Crystal structure of heat shock factor1-DBD complex with ds-DNA and TtT

Released:
DOI: 10.2210/pdb5hdn/pdb
PDB entry 5hdn coloured by chain, front view.
PDB entry 5hdn coloured by chain, side view.
PDB entry 5hdn coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Feng H, Liu W, Wang DC

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-118736 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Heat shock factor protein 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 112 amino acids
Theoretical weight: 13.16 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q00613 (Residues: 15-120; Coverage: 20%)
Gene names: HSF1, HSTF1
Sequence domains: HSF-type DNA-binding
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
DNA (5'-D(*GP*GP*TP*TP*CP*TP*AP*GP*AP*AP*CP*C)-3') Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 12 nucleotides
Theoretical weight: 3.66 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens

Ligands and Environments

2 bound ligands:
Ligand CIT 1 x CIT
Ligand NA 3 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P21
Unit cell:
a: 39.415Å b: 127.367Å c: 55.268Å
α: 90° β: 100.55° γ: 90°
R-values:
R R work R free
0.176 0.174 0.202
Expression systems:
  • Escherichia coli
  • Homo sapiens

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