› 5hhm

X-ray diffraction
2.5Å resolution

Crystal Structure of the JM22 TCR in complex with HLA-A*0201 in complex with M1-F5L

Released:
DOI: 10.2210/pdb5hhm/pdb
PDB entry 5hhm coloured by chain, front view.
PDB entry 5hhm coloured by chain, side view.
PDB entry 5hhm coloured by chain, top view.
Source organisms:
Primary publication:
Molecular basis for universal HLA-A*0201-restricted CD8+ T-cell immunity against influenza viruses.
Valkenburg SA, Josephs TM, Clemens EB, Grant EJ, Nguyen TH, Wang GC, Price DA, Miller A, Tong SY, Thomas PG, Doherty PC, Rossjohn J, Gras S, Kedzierska K
Proc Natl Acad Sci U S A 113 4440-5 (2016)
PMID: 27036003

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-100456 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
HLA class I histocompatibility antigen, A alpha chain Chains: A, F
Molecule details ›
Chains: A, F
Length: 276 amino acids
Theoretical weight: 31.95 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P04439 (Residues: 25-299; Coverage: 81%)
Gene names: HLA-A, HLAA
Sequence domains:
Structure domains: MHC class I-like antigen recognition-like
Beta-2-microglobulin Chains: B, G
Molecule details ›
Chains: B, G
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
M1-F5L, GILGLVFTL Chains: C, H
Molecule details ›
Chains: C, H
Length: 9 amino acids
Theoretical weight: 932 Da
Source organism: synthetic construct
Expression system: Not provided
T cell receptor alpha chain constant; T cell receptor alpha variable 27 Chains: D, I
Molecule details ›
Chains: D, I
Length: 200 amino acids
Theoretical weight: 21.87 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A087WT01 (Residues: 20-109; Coverage: 100%)
  • Canonical: P01848 (Residues: 1-17, 18-89; Coverage: 64%)
Gene names: TCRA, TRAC, TRAV27
Sequence domains:
Structure domains: Immunoglobulins
T cell receptor beta constant 2; T cell receptor beta variable 19 Chains: E, J
Molecule details ›
Chains: E, J
Length: 241 amino acids
Theoretical weight: 27.42 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A075B6N1 (Residues: 21-113; Coverage: 99%)
  • Canonical: A0A5B9 (Residues: 1-129; Coverage: 73%)
Gene names: TCRBC2, TRBC2, TRBV19
Sequence domains:
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: C2
Unit cell:
a: 242.876Å b: 47.296Å c: 185.945Å
α: 90° β: 115.49° γ: 90°
R-values:
R R work R free
0.22 0.216 0.289
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

14 review citations

Harnessing the Power of T Cells: The Promising Hope for a Universal Influenza Vaccine.
Clemens et al. (2018)
13 more

5 mentions without citation

Molecular basis for universal HLA-A*0201-restricted CD8+ T-cell immunity against influenza viruses.
Valkenburg et al. (2016)
4 more