› 5hjb

X-ray diffraction
2.7Å resolution

AF9 YEATS in complex with histone H3 Crotonylation at K9

Released:
DOI: 10.2210/pdb5hjb/pdb
PDB entry 5hjb coloured by chain, front view.
PDB entry 5hjb coloured by chain, side view.
PDB entry 5hjb coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular Coupling of Histone Crotonylation and Active Transcription by AF9 YEATS Domain.
Li Y, Sabari BR, Panchenko T, Wen H, Zhao D, Guan H, Wan L, Huang H, Tang Z, Zhao Y, Roeder RG, Shi X, Allis CD, Li H
Mol Cell 62 181-193 (2016)
PMID: 27105114

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154673 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protein AF-9 Chain: A
Molecule details ›
Chain: A
Length: 140 amino acids
Theoretical weight: 16.55 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P42568 (Residues: 1-138; Coverage: 24%)
Gene names: AF9, MLLT3, YEATS3
Sequence domains: YEATS family
Structure domains: YEATS domain
Histone H3.1 Chain: B
Molecule details ›
Chain: B
Length: 8 amino acids
Theoretical weight: 989 Da
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P68431 (Residues: 4-11; Coverage: 6%)
Gene names: H3C1, H3C10, H3C11, H3C12, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3FA, H3FB, H3FC HIST1H3C, H3FD, H3FF, H3FH, H3FI, H3FJ, H3FK, H3FL, HIST1H3A, HIST1H3B, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J

Ligands and Environments

No bound ligands
1 modified residue:
Ligand KCR 1 x KCR

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P3221
Unit cell:
a: 97.595Å b: 97.595Å c: 39.685Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.21 0.206 0.253
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

56 review citations

Functions of bromodomain-containing proteins and their roles in homeostasis and cancer.
Fujisawa et al. (2017)
55 more

16 mentions without citation

Metabolic regulation of gene expression through histone acylations.
Sabari et al. (2017)
15 more