› 5hoo

X-ray diffraction
3.3Å resolution

Crystal structure of the Mos1 Strand Transfer Complex

Released:
DOI: 10.2210/pdb5hoo/pdb
PDB entry 5hoo coloured by chain, front view.
PDB entry 5hoo coloured by chain, side view.
PDB entry 5hoo coloured by chain, top view.
Source organisms:
Primary publication:
A bend, flip and trap mechanism for transposon integration.
Morris ER, Grey H, McKenzie G, Jones AC, Richardson JM
Elife 5 (2016)
PMID: 27223327

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-118748 (preferred)
Entry contents:
1 distinct polypeptide molecule
3 distinct DNA molecules
Macromolecules (4 distinct):
Mariner Mos1 transposase Chains: A, B
Molecule details ›
Chains: A, B
Length: 345 amino acids
Theoretical weight: 40.87 KDa
Source organism: Drosophila mauritiana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q7JQ07 (Residues: 1-345; Coverage: 100%)
Gene name: mariner\T
Sequence domains:
Structure domains:
Mos1 IR DNA NTS Chains: C, E
Molecule details ›
Chains: C, E
Length: 25 nucleotides
Theoretical weight: 7.77 KDa
Source organism: synthetic construct
Expression system: Not provided
Mos1 IR TS joined to Target DNA,Mos1 IR TS joined to Target DNA Chains: D, F
Molecule details ›
Chains: D, F
Length: 36 nucleotides
Theoretical weight: 11.04 KDa
Source organism: Drosophila mauritiana
Expression system: Not provided
Target DNA Chains: G, H
Molecule details ›
Chains: G, H
Length: 10 nucleotides
Theoretical weight: 3.01 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: C2
Unit cell:
a: 256.34Å b: 58.83Å c: 110.14Å
α: 90° β: 94.91° γ: 90°
R-values:
R R work R free
0.234 0.232 0.274
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

4 review citations

Integration site selection by retroviruses and transposable elements in eukaryotes.
Sultana et al. (2017)
3 more

5 mentions without citation

Jump ahead with a twist: DNA acrobatics drive transposition forward.
Arinkin et al. (2019)
4 more