› 5hzu

X-ray diffraction
1.89Å resolution

Crystal structure of Dronpa-Ni2+

Released:
DOI: 10.2210/pdb5hzu/pdb
PDB entry 5hzu coloured by chain, front view.
PDB entry 5hzu coloured by chain, side view.
PDB entry 5hzu coloured by chain, top view.
Source organism: Echinophyllia sp. SC22
Primary publication:
Crystal structures of Dronpa complexed with quenchable metal ions provide insight into metal biosensor development.
Kim IJ, Kim S, Park J, Eom I, Kim S, Kim JH, Ha SC, Kim YG, Hwang KY, Nam KH
FEBS Lett 590 2982-90 (2016)
PMID: 27433793

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178339 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fluorescent protein Dronpa Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 215 amino acids
Theoretical weight: 24.65 KDa
Source organism: Echinophyllia sp. SC22
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5TLG6 (Residues: 2-218; Coverage: 97%)
Gene name: Dronpa
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

1 bound ligand:
Ligand NI 25 x NI
1 modified residue:
Ligand GYS 12 x GYS

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 7A (6B, 6C1)
Spacegroup: P1
Unit cell:
a: 105.372Å b: 109.403Å c: 72.087Å
α: 71.11° β: 85.97° γ: 61.3°
R-values:
R R work R free
0.153 0.151 0.199
Expression system: Escherichia coli BL21(DE3)

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Citations

7 citation in other articles

Spectroscopic Analysis of the Cu2+-Induced Fluorescence Quenching of Fluorescent Proteins AmCyan and mOrange2.
Bae et al. (2018)
6 more

1 mention without citation

Self-association of a highly charged arginine-rich cell-penetrating peptide.
Tesei et al. (2017)