› 5i0w

X-ray diffraction
1.55Å resolution

IRON AND COPPER-BOUND P19 FROM CAMPYLOBACTER JEJUNI UNDER REDUCING CONDITIONS

Released:
DOI: 10.2210/pdb5i0w/pdb
PDB entry 5i0w coloured by chain, front view.
PDB entry 5i0w coloured by chain, side view.
PDB entry 5i0w coloured by chain, top view.
Source organism: Campylobacter jejuni subsp. jejuni 81-176
Primary publication:
A copper site is required for iron transport by the periplasmic proteins P19 and FetP.
Chan ACK, Lin H, Koch D, Grass G, Nies DH, Murphy MEP
Metallomics 12 1530-1541 (2020)
PMID: 32780051

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102177 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Iron transporter Chains: A, B
Molecule details ›
Chains: A, B
Length: 159 amino acids
Theoretical weight: 17.59 KDa
Source organism: Campylobacter jejuni subsp. jejuni 81-176
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0H3PA01 (Residues: 22-179; Coverage: 100%)
Gene name: CJJ81176_1650
Sequence domains: Fe2+ transport protein
Structure domains: Periplasmic metal-binding protein Tp34-type

Ligands and Environments

4 bound ligands:
Ligand TAM 1 x TAM
Ligand CU1 2 x CU1
Ligand CL 2 x CL
Ligand FE2 1 x FE2
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P21212
Unit cell:
a: 53.911Å b: 73.229Å c: 74.929Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.177 0.209
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 citations in other articles

Dissecting components of the Campylobacter jejuni fetMP-fetABCDEF gene cluster under iron limitation.
Richardson-Sanchez et al. (2024)