› 5i0y

X-ray diffraction
1.4Å resolution

COPPER-BOUND E46Q VARIANT OF UROPATHOGENIC ESCHERICHIA COLI STRAIN F11 FETP

Released:
DOI: 10.2210/pdb5i0y/pdb
PDB entry 5i0y coloured by chain, front view.
PDB entry 5i0y coloured by chain, side view.
PDB entry 5i0y coloured by chain, top view.
Source organism: Escherichia coli F11
Primary publication:
A copper site is required for iron transport by the periplasmic proteins P19 and FetP.
Chan ACK, Lin H, Koch D, Grass G, Nies DH, Murphy MEP
Metallomics 12 1530-1541 (2020)
PMID: 32780051

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-103707 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Periplasmic protein-probably involved in high-affinity Fe2+ transport Chains: A, B
Molecule details ›
Chains: A, B
Length: 160 amino acids
Theoretical weight: 17.66 KDa
Source organism: Escherichia coli F11
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A1S4NYE7 (Residues: 1-1, 3-153; Coverage: 95%)
Gene name: EcF11_1994
Sequence domains: Fe2+ transport protein
Structure domains: Periplasmic metal-binding protein Tp34-type

Ligands and Environments

1 bound ligand:
Ligand CU 2 x CU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: C2
Unit cell:
a: 83.16Å b: 36.54Å c: 101Å
α: 90° β: 106.52° γ: 90°
R-values:
R R work R free
0.19 0.188 0.214
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 citations in other articles

Dissecting components of the Campylobacter jejuni fetMP-fetABCDEF gene cluster under iron limitation.
Richardson-Sanchez et al. (2024)