› 5ibx

X-ray diffraction
1.65Å resolution

1.65 Angstrom Crystal Structure of Triosephosphate Isomerase (TIM) from Streptococcus pneumoniae

Released:
DOI: 10.2210/pdb5ibx/pdb
Model geometry
Fit model/data
PDB entry 5ibx coloured by chain, front view.
PDB entry 5ibx coloured by chain, side view.
PDB entry 5ibx coloured by chain, top view.
Source organism: Streptococcus pneumoniae D39
Entry authors: Minasov G, Shuvalova L, Dubrovska I, Flores K, Shatsman S, Kwon K, Anderson WF, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
D-glyceraldehyde 3-phosphate = glycerone phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-170106 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Triosephosphate isomerase Chains: A, B, D, E, H
Molecule details ›
Chains: A, B, D, E, H
Length: 255 amino acids
Theoretical weight: 26.92 KDa
Source organism: Streptococcus pneumoniae D39
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q04JH4 (Residues: 1-252; Coverage: 100%)
Gene names: SPD_1404, tpiA
Sequence domains: Triosephosphate isomerase
Structure domains: Aldolase class I
Triosephosphate isomerase Chains: C, F, G
Molecule details ›
Chains: C, F, G
Length: 255 amino acids
Theoretical weight: 26.85 KDa
Source organism: Streptococcus pneumoniae D39
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q04JH4 (Residues: 1-252; Coverage: 100%)
Gene names: SPD_1404, tpiA
Sequence domains: Triosephosphate isomerase
Structure domains: Aldolase class I

Ligands and Environments

1 bound ligand:
Ligand NA 13 x NA
1 modified residue:
Ligand CME 5 x CME

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P1
Unit cell:
a: 78.845Å b: 84.351Å c: 92.463Å
α: 75.43° β: 65.97° γ: 62.03°
R-values:
R R work R free
0.198 0.196 0.232
Expression system: Escherichia coli BL21(DE3)

Quick links