› 5id6

X-ray diffraction
2.38Å resolution

Structure of Cpf1/RNA Complex

Released:
DOI: 10.2210/pdb5id6/pdb
PDB entry 5id6 coloured by chain, front view.
PDB entry 5id6 coloured by chain, side view.
PDB entry 5id6 coloured by chain, top view.
Source organisms:
Primary publication:
The crystal structure of Cpf1 in complex with CRISPR RNA.
Dong D, Ren K, Qiu X, Zheng J, Guo M, Guan X, Liu H, Li N, Zhang B, Yang D, Ma C, Wang S, Wu D, Ma Y, Fan S, Wang J, Gao N, Huang Z
Nature 532 522-6 (2016)
PMID: 27096363

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-103177 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Cpf1 Chain: A
Molecule details ›
Chain: A
Length: 1228 amino acids
Theoretical weight: 144.13 KDa
Source organism: Lachnospiraceae bacterium ND2006
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A182DWE3 (Residues: 1-1228; Coverage: 100%)
Sequence domains:
RNA (5'-R(P*AP*AP*UP*UP*UP*CP*UP*AP*CP*UP*AP*AP*GP*UP*GP*UP*AP*GP*AP*UP*C)-3') Chain: G
Molecule details ›
Chain: G
Length: 21 nucleotides
Theoretical weight: 6.66 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MG 2 x MG
1 modified residue:
Ligand MSE 19 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: C2
Unit cell:
a: 168.352Å b: 83.011Å c: 124.086Å
α: 90° β: 106.72° γ: 90°
R-values:
R R work R free
0.205 0.202 0.261
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

50 review citations

Diversity, classification and evolution of CRISPR-Cas systems.
Koonin et al. (2017)
49 more

12 mentions without citation

HIV infection detection using CRISPR/Cas systems: Present and future prospects.
Deng et al. (2023)
11 more