› 5ify

X-ray diffraction
2.25Å resolution

Crystal structure of Glucose-1-phosphate thymidylyltransferase from Burkholderia vietnamiensis in complex with 2 -Deoxyuridine-5'-monophosphate and 2'-Deoxy-Thymidine-B-L-Rhamnose

Released:
DOI: 10.2210/pdb5ify/pdb
Model geometry
Fit model/data
PDB entry 5ify coloured by chain, front view.
PDB entry 5ify coloured by chain, side view.
PDB entry 5ify coloured by chain, top view.
Source organism: Burkholderia vietnamiensis G4
Entry author: Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reaction catalysed: 
dTTP + alpha-D-glucose 1-phosphate = diphosphate + dTDP-alpha-D-glucose
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107357 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glucose-1-phosphate thymidylyltransferase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 305 amino acids
Theoretical weight: 34.07 KDa
Source organism: Burkholderia vietnamiensis G4
Expression system: Escherichia coli
UniProt:
  • Canonical: A4JC15 (Residues: 1-297; Coverage: 100%)
Gene name: Bcep1808_0806
Sequence domains: Nucleotidyl transferase
Structure domains: Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A

Ligands and Environments

2 bound ligands:
Ligand TRH 6 x TRH
Ligand UMP 6 x UMP
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P21212
Unit cell:
a: 109.6Å b: 254.72Å c: 82.05Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.158 0.157 0.197
Expression system: Escherichia coli

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