› 5ih1

X-ray diffraction
1.31Å resolution

Macrolide 2'-phosphotransferase type II - complex with GDP and phosphorylated josamycin

Released:
DOI: 10.2210/pdb5ih1/pdb
PDB entry 5ih1 coloured by chain, front view.
PDB entry 5ih1 coloured by chain, side view.
PDB entry 5ih1 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structural Basis for Kinase-Mediated Macrolide Antibiotic Resistance.
Fong DH, Burk DL, Blanchet J, Yan AY, Berghuis AM
Structure 25 750-761.e5 (2017)
PMID: 28416110

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128457 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aminoglycoside phosphotransferase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 302 amino acids
Theoretical weight: 34.53 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O32553 (Residues: 1-302; Coverage: 100%)
Gene names: HVY77_26855, mph(B), mphB, pO103_99
Sequence domains: Phosphotransferase enzyme family

Ligands and Environments

4 bound ligands:
Ligand GDP 1 x GDP
Ligand MG 1 x MG
Ligand CA 1 x CA
Ligand 6BQ 1 x 6BQ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P212121
Unit cell:
a: 40.37Å b: 80.87Å c: 97.87Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.162 0.161 0.178
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

The macrolide antibiotic renaissance.
Dinos GP. (2017)
6 more