› 5ivd

X-ray diffraction
1.71Å resolution

The alpha-esterase-7 carboxylesterase, E3, from the blowfly Lucilia cuprina: apo-enzyme qFit multi-conformer model

Released:
DOI: 10.2210/pdb5ivd/pdb
PDB entry 5ivd coloured by chain, front view.
PDB entry 5ivd coloured by chain, side view.
PDB entry 5ivd coloured by chain, top view.
Source organism: Lucilia cuprina
Primary publication:
Mapping the Accessible Conformational Landscape of an Insect Carboxylesterase Using Conformational Ensemble Analysis and Kinetic Crystallography.
Correy GJ, Carr PD, Meirelles T, Mabbitt PD, Fraser NJ, Weik M, Jackson CJ
Structure 24 977-87 (2016)
PMID: 27210287

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173217 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carboxylic ester hydrolase Chain: A
Molecule details ›
Chain: A
Length: 577 amino acids
Theoretical weight: 66.39 KDa
Source organism: Lucilia cuprina
Expression system: Escherichia coli
UniProt:
  • Canonical: Q25252 (Residues: 1-570; Coverage: 100%)
Gene name: LcaE7
Sequence domains: Carboxylesterase family
Structure domains: alpha/beta hydrolase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: C2221
Unit cell:
a: 48.574Å b: 100.472Å c: 221.715Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.178 0.23
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Identifying and Visualizing Macromolecular Flexibility in Structural Biology.
Palamini et al. (2016)
2 more