› 5iws

X-ray diffraction
2.55Å resolution

Crystal structure of the transporter MalT, the EIIC domain from the maltose-specific phosphotransferase system

Released:
DOI: 10.2210/pdb5iws/pdb
PDB entry 5iws coloured by chain, front view.
PDB entry 5iws coloured by chain, side view.
PDB entry 5iws coloured by chain, top view.
Source organism: Bacillus cereus E33L
Primary publication:
The Structure of a Sugar Transporter of the Glucose EIIC Superfamily Provides Insight into the Elevator Mechanism of Membrane Transport.
McCoy JG, Ren Z, Stanevich V, Lee J, Mitra S, Levin EJ, Poget S, Quick M, Im W, Zhou M
Structure (2016)
PMID: 27161976

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179042 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Protein-N(Pi)-phosphohistidine-sugar phosphotransferase (Enzyme II of the phosphotransferase system) (PTS system glucose-specific IIBC component) Chain: A
Molecule details ›
Chain: A
Length: 470 amino acids
Theoretical weight: 51.71 KDa
Source organism: Bacillus cereus E33L
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q63GK8 (Residues: 3-472; Coverage: 86%)
Gene names: BCE33L0344, ptsG
Sequence domains: Phosphotransferase system, EIIC

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
No bound ligands
1 modified residue:
Ligand MSE 16 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: I222
Unit cell:
a: 71.633Å b: 108.182Å c: 139.329Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.242 0.237 0.282
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Transporters of glucose and other carbohydrates in bacteria.
Jeckelmann et al. (2020)
4 more

7 mentions without citation

Elevator-type mechanisms of membrane transport.
Garaeva et al. (2020)
6 more