› 5j1t

X-ray diffraction
1.4Å resolution

TorsinAdeltaE-LULL1 complex, H. sapiens, bound to VHH-BS2

Released:
DOI: 10.2210/pdb5j1t/pdb
PDB entry 5j1t coloured by chain, front view.
PDB entry 5j1t coloured by chain, side view.
PDB entry 5j1t coloured by chain, top view.
Source organisms:
Primary publication:
Structures of TorsinA and its disease-mutant complexed with an activator reveal the molecular basis for primary dystonia.
Demircioglu FE, Sosa BA, Ingram J, Ploegh HL, Schwartz TU
Elife 5 (2016)
PMID: 27490483

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-208022 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Torsin-1A Chain: A
Molecule details ›
Chain: A
Length: 283 amino acids
Theoretical weight: 32.61 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O14656 (Residues: 51-332; Coverage: 90%)
Gene names: DQ2, DYT1, TA, TOR1A, TORA
Sequence domains:
Torsin-1A-interacting protein 2 Chain: B
Molecule details ›
Chain: B
Length: 239 amino acids
Theoretical weight: 26.73 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8NFQ8 (Residues: 233-470; Coverage: 51%)
Gene names: IFRG15, LULL1, TOR1AIP2
Sequence domains: Lamina-associated polypeptide 1, AAA+ activator domain
Structure domains: Rossmann fold
VHH domain BS-2 Chain: C
Molecule details ›
Chain: C
Length: 123 amino acids
Theoretical weight: 13.3 KDa
Source organism: Vicugna pacos
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

4 bound ligands:
Ligand ATP 1 x ATP
Ligand MG 1 x MG
Ligand CL 1 x CL
Ligand BTB 2 x BTB
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 75.435Å b: 88.445Å c: 105.331Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.148 0.148 0.177
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Update on the Genetics of Dystonia.
Lohmann et al. (2017)
8 more

4 mentions without citation

Exploring cellular biochemistry with nanobodies.
Cheloha et al. (2020)
3 more