› 5j63

X-ray diffraction
2.5Å resolution

Crystal Structure of the N-terminal N-formyltransferase Domain (residues 1-306) of Escherichia coli Arna in Complex with UDP-Ara4N and Folinic Acid

Released:
DOI: 10.2210/pdb5j63/pdb
PDB entry 5j63 coloured by chain, front view.
PDB entry 5j63 coloured by chain, side view.
PDB entry 5j63 coloured by chain, top view.
Source organism: Escherichia coli H736
Primary publication:
Structure of the Escherichia coli ArnA N-formyltransferase domain in complex with N(5) -formyltetrahydrofolate and UDP-Ara4N.
Genthe NA, Thoden JB, Holden HM
Protein Sci 25 1555-62 (2016)
PMID: 27171345

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-165848 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bifunctional polymyxin resistance protein ArnA Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 308 amino acids
Theoretical weight: 33.53 KDa
Source organism: Escherichia coli H736
Expression system: Escherichia coli
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand G3N 4 x G3N
Ligand FNX 4 x FNX
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P1
Unit cell:
a: 75.994Å b: 76.206Å c: 84.852Å
α: 89.94° β: 63.78° γ: 61.54°
R-values:
R R work R free
0.216 0.212 0.284
Expression system: Escherichia coli

Quick links

Citations

6 citation in other articles

Investigation of a sugar N-formyltransferase from the plant pathogen Pantoea ananatis.
Hofmeister et al. (2019)
5 more

1 mention without citation

Structure of the Escherichia coli ArnA N-formyltransferase domain in complex with N(5) -formyltetrahydrofolate and UDP-Ara4N.
Genthe et al. (2016)