› 5jk9

X-ray diffraction
2.1Å resolution

Crystal structure of human IZUMO1

Released:
DOI: 10.2210/pdb5jk9/pdb
PDB entry 5jk9 coloured by chain, front view.
PDB entry 5jk9 coloured by chain, side view.
PDB entry 5jk9 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of IZUMO1-JUNO reveals sperm-oocyte recognition during mammalian fertilization.
Ohto U, Ishida H, Krayukhina E, Uchiyama S, Inoue N, Shimizu T
Nature 534 566-9 (2016)
PMID: 27309808

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184956 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Izumo sperm-egg fusion protein 1 Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 246 amino acids
Theoretical weight: 28.16 KDa
Source organism: Homo sapiens
Expression system: Drosophila
UniProt:
  • Canonical: Q8IYV9 (Residues: 22-255; Coverage: 71%)
Gene name: IZUMO1
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand NAG 6 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NE3A
Spacegroup: P1
Unit cell:
a: 64.936Å b: 75.067Å c: 108.885Å
α: 77.65° β: 79.11° γ: 70.66°
R-values:
R R work R free
0.196 0.194 0.231
Expression system: Drosophila

Quick links

Citations

23 review citations

The hallmarks of cell-cell fusion.
Hernández et al. (2017)
22 more

3 mentions without citation

Chemistry and Enzymology of Disulfide Cross-Linking in Proteins.
Fass et al. (2018)
2 more