› 5jkj

X-ray diffraction
1.55Å resolution

Crystal structure of esterase E22 L374D mutant

Released:
DOI: 10.2210/pdb5jkj/pdb
PDB entry 5jkj coloured by chain, front view.
PDB entry 5jkj coloured by chain, side view.
PDB entry 5jkj coloured by chain, top view.
Source organism: uncultured bacterium
Entry authors: Zhang Y, Wang P, Yao Q

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-103248 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable acyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 370 amino acids
Theoretical weight: 40.44 KDa
Source organism: uncultured bacterium
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: A0A1B1H1K0 (Residues: 104-473; Coverage: 77%)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P21
Unit cell:
a: 58.619Å b: 68.898Å c: 82.524Å
α: 90° β: 91.11° γ: 90°
R-values:
R R work R free
0.153 0.151 0.18
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

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