› 5jlf

Electron Microscopy
3.6Å resolution

Structure of the F-actin-tropomyosin complex (Reprocessed)

Released:
DOI: 10.2210/pdb5jlf/pdb
PDB entry 5jlf coloured by chain, front view.
PDB entry 5jlf coloured by chain, side view.
PDB entry 5jlf coloured by chain, top view.
Source organisms:
Primary publication:
Cryo-EM structure of a human cytoplasmic actomyosin complex at near-atomic resolution.
von der Ecken J, Heissler SM, Pathan-Chhatbar S, Manstein DJ, Raunser S
Nature 534 724-8 (2016)
PMID: 27324845
Related structures: EMD-8162

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159330 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Actin, alpha skeletal muscle Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 375 amino acids
Theoretical weight: 41.88 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P68135 (Residues: 3-377; Coverage: 100%)
Gene names: ACTA, ACTA1
Sequence domains: Actin
Structure domains:
Tropomyosin Alpha-1 Chains: F, G
Molecule details ›
Chains: F, G
Length: 135 amino acids
Theoretical weight: 11.51 KDa
Source organism: Mus musculus
Expression system: Escherichia coli

Ligands and Environments

2 bound ligands:
Ligand ADP 5 x ADP
Ligand MG 5 x MG
1 modified residue:
Ligand HIC 5 x HIC

Experiments and Validation Details

Entry percentile scores
Resolution: 3.6Å
Relevant EMDB volumes: EMD-8162
Expression system: Escherichia coli

Quick links

Citations

26 review citations

How Myosin Generates Force on Actin Filaments.
Houdusse et al. (2016)
25 more

20 mentions without citation

Towards a structural understanding of the remodeling of the actin cytoskeleton.
Merino et al. (2020)
19 more