Function and Biology Details
Reaction catalysed:
Limited hydrolysis of proteins of the neuroexocytosis apparatus, synaptobrevin (also known as neuronal vesicle-associated membrane protein, VAMP), synaptosome-associated protein of 25 kDa (SNAP25) or syntaxin. No detected action on small molecule substrates.
Biochemical function:
- not assigned
Biological process:
- not assigned
Cellular component:
Structure analysis Details
Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-144090 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Botulinum neurotoxin A heavy chain
Molecule details ›
Chains: A, C, E, G
Length: 433 amino acids
Theoretical weight: 50.47 KDa
Source organism: Clostridium botulinum
Expression system: Escherichia coli
UniProt:
Sequence domains:
Length: 433 amino acids
Theoretical weight: 50.47 KDa
Source organism: Clostridium botulinum
Expression system: Escherichia coli
UniProt:
- Canonical:
P0DPI1 (Residues: 872-1296; Coverage: 33%)
Sequence domains:
- Clostridium neurotoxin, N-terminal receptor binding
- Clostridium neurotoxin, C-terminal receptor binding
Synaptic vesicle glycoprotein 2C
Molecule details ›
Chains: B, D, F, H
Length: 124 amino acids
Theoretical weight: 14.52 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
Sequence domains: Pentapeptide repeats (9 copies)
Structure domains: E3 ubiquitin-protein ligase SopA
Length: 124 amino acids
Theoretical weight: 14.52 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
- Canonical: Q9Z2I6 (Residues: 455-577; Coverage: 17%)
Sequence domains: Pentapeptide repeats (9 copies)
Structure domains: E3 ubiquitin-protein ligase SopA
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
APS BEAMLINE 24-ID-E
Spacegroup:
P21
Expression system: Escherichia coli
