› 5jrb

X-ray diffraction
2.4Å resolution

Rad52(1-212) K102A/K133A/E202A mutant

Released:
DOI: 10.2210/pdb5jrb/pdb
PDB entry 5jrb coloured by chain, front view.
PDB entry 5jrb coloured by chain, side view.
PDB entry 5jrb coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the human DNA-repair protein RAD52 containing surface mutations.
Saotome M, Saito K, Onodera K, Kurumizaka H, Kagawa W
Acta Crystallogr F Struct Biol Commun 72 598-603 (2016)
PMID: 27487923

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo undecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155038 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA repair protein RAD52 homolog Chains: A, B, C, D, E, F, G, H, I, J, K
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K
Length: 215 amino acids
Theoretical weight: 23.54 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P43351 (Residues: 1-212; Coverage: 51%)
Gene name: RAD52
Sequence domains: Rad52/22 family double-strand break repair protein
Structure domains: DNA repair protein Rad52/59/22

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: P21
Unit cell:
a: 110.955Å b: 98.516Å c: 116.367Å
α: 90° β: 91.99° γ: 90°
R-values:
R R work R free
0.226 0.224 0.273
Expression system: Escherichia coli

Quick links

Citations

2 citation in other articles

Structural Basis of Homology-Directed DNA Repair Mediated by RAD52.
Saotome et al. (2018)
1 more

5 mentions without citation

Novel Insights into RAD52's Structure, Function, and Druggability for Synthetic Lethality and Innovative Anticancer Therapies.
Balboni et al. (2023)
4 more