Function and Biology Details
Reaction catalysed:
A phosphate monoester + H(2)O = an alcohol + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure analysis Details
Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132775 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protein-export protein SecB
Molecule details ›
Chains: A, B, C, D
Length: 155 amino acids
Theoretical weight: 17.29 KDa
Source organism: Escherichia coli O157:H7
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains: Preprotein translocase subunit SecB
Structure domains: SecB-like
Length: 155 amino acids
Theoretical weight: 17.29 KDa
Source organism: Escherichia coli O157:H7
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical:
P0AG86 (Residues: 1-155; Coverage: 100%)
Sequence domains: Preprotein translocase subunit SecB
Structure domains: SecB-like
Alkaline phosphatase
Molecule details ›
Chains: E, F, G, H
Length: 22 amino acids
Theoretical weight: 2.4 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains: Alkaline phosphatase
Length: 22 amino acids
Theoretical weight: 2.4 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical: P00634 (Residues: 450-471; Coverage: 5%)
Sequence domains: Alkaline phosphatase
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
Chemical shift assignment:
14%
Refinement method:
molecular dynamics
Expression system: Escherichia coli BL21(DE3)
