› 5jug

X-ray diffraction
0.96Å resolution

Structure of an inactive (E45Q) variant of a beta-1,4-mannanase, SsGH134, in complex with Man5

Released:
DOI: 10.2210/pdb5jug/pdb
PDB entry 5jug coloured by chain, front view.
PDB entry 5jug coloured by chain, side view.
PDB entry 5jug coloured by chain, top view.
Source organism: Streptomyces sp. NRRL B-16215
Primary publication:
A β-Mannanase with a Lysozyme-like Fold and a Novel Molecular Catalytic Mechanism.
Jin Y, Petricevic M, John A, Raich L, Jenkins H, Portela De Souza L, Cuskin F, Gilbert HJ, Rovira C, Goddard-Borger ED, Williams SJ, Davies GJ
ACS Cent Sci 2 896-903 (2016)
PMID: 28058278

Function and Biology Details

Reaction catalysed: 
Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-103530 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Beta-1,4-mannanase Chain: A
Molecule details ›
Chain: A
Length: 168 amino acids
Theoretical weight: 17.59 KDa
Source organism: Streptomyces sp. NRRL B-16215
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A1L1QK16 (Residues: 1-168; Coverage: 100%)
Sequence domains: Glycosyl hydrolase family 134

Ligands and Environments

Carbohydrate polymer : NEW Components: BMA
Carbohydrate polymer
3 bound ligands:
Ligand MAN 1 x MAN
Ligand GOL 1 x GOL
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P212121
Unit cell:
a: 36.707Å b: 53.841Å c: 82.636Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.123 0.122 0.141
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Enzymatic Transition States and Drug Design.
Schramm VL. (2018)
1 more

3 mentions without citation

A β-Mannanase with a Lysozyme-like Fold and a Novel Molecular Catalytic Mechanism.
Jin et al. (2016)
2 more