› 5k58

X-ray diffraction
2.77Å resolution

Structure of the K. pneumonia SlmA-DNA complex bound to the C-terminal of the cell division protein FtsZ

Released:
DOI: 10.2210/pdb5k58/pdb
PDB entry 5k58 coloured by chain, front view.
PDB entry 5k58 coloured by chain, side view.
PDB entry 5k58 coloured by chain, top view.
Source organisms:
Primary publication:
Structures of the nucleoid occlusion protein SlmA bound to DNA and the C-terminal domain of the cytoskeletal protein FtsZ.
Schumacher MA, Zeng W
Proc Natl Acad Sci U S A 113 4988-93 (2016)
PMID: 27091999

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-118890 (preferred)
Entry contents:
2 distinct polypeptide molecules
1 distinct DNA molecule
Macromolecules (3 distinct):
Nucleoid occlusion factor SlmA Chains: A, B, E, F
Molecule details ›
Chains: A, B, E, F
Length: 190 amino acids
Theoretical weight: 22 KDa
Source organism: Escherichia coli O139:H28 str. E24377A
Expression system: Escherichia coli
UniProt:
  • Canonical: P0C093 (Residues: 9-198; Coverage: 96%)
Gene names: JW5641, b3641, slmA, ttk, yicB
Sequence domains: Bacterial regulatory proteins, tetR family
Structure domains: Tetracycline Repressor, domain 2
Cell division protein FtsZ Chains: K, L, M, N
Molecule details ›
Chains: K, L, M, N
Length: 8 amino acids
Theoretical weight: 945 Da
Source organism: synthetic construct
Expression system: Not provided
UniProt:
  • Canonical: P0A9A6 (Residues: 372-379; Coverage: 2%)
Gene names: JW0093, b0095, ftsZ, sfiB, sulB
DNA (5'-D(*GP*TP*GP*AP*GP*TP*AP*CP*TP*CP*AP*C)-3') Chains: R, T
Molecule details ›
Chains: R, T
Length: 12 nucleotides
Theoretical weight: 3.66 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P32
Unit cell:
a: 84.8Å b: 84.8Å c: 161.6Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.245 0.247 0.268
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

8 review citations

At the Heart of Bacterial Cytokinesis: The Z Ring.
Du et al. (2019)
7 more