› 5k78

X-ray diffraction
2.64Å resolution

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111331 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct RNA molecules
Macromolecules (3 distinct):
Lariat debranching enzyme Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 360 amino acids
Theoretical weight: 41.85 KDa
Source organism: Entamoeba histolytica
Expression system: Escherichia coli
UniProt:
  • Canonical: C4M1P9 (Residues: 1-354; Coverage: 100%)
Gene names: DBR1, EHI_062730
Sequence domains:
branch 1 of branched RNA 5'-UACUAA(2'-GUAUGU)CAAGU-3' Chains: X, Y
Molecule details ›
Chains: X, Y
Length: 11 nucleotides
Theoretical weight: 3.48 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided
branch 2 of branched RNA 5'-UACUAA(2'-GUAUGU)CAAGU-3' Chains: x, y
Molecule details ›
Chains: x, y
Length: 6 nucleotides
Theoretical weight: 1.89 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided

Ligands and Environments

3 bound ligands:
Ligand FE2 5 x FE2
Ligand ZN 5 x ZN
Ligand SO4 13 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 73.893Å b: 142.673Å c: 215.131Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.195 0.246
Expression systems:
  • Escherichia coli
  • Not provided

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