› 5k7s

Electron crystallography
1.6Å resolution

MicroED structure of proteinase K at 1.6 A resolution

Released:
DOI: 10.2210/pdb5k7s/pdb
PDB entry 5k7s coloured by chain, front view.
PDB entry 5k7s coloured by chain, side view.
PDB entry 5k7s coloured by chain, top view.
Source organism: Parengyodontium album
Primary publication:
Atomic-resolution structures from fragmented protein crystals with the cryoEM method MicroED.
de la Cruz MJ, Hattne J, Shi D, Seidler P, Rodriguez J, Reyes FE, Sawaya MR, Cascio D, Weiss SC, Kim SK, Hinck CS, Hinck AP, Calero G, Eisenberg D, Gonen T
Nat Methods 14 399-402 (2017)
PMID: 28192420
Related structures: EMD-8221

Function and Biology Details

Reaction catalysed: 
Hydrolysis of keratin, and of other proteins with subtilisin-like specificity. Hydrolyzes peptide amides.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139275 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Proteinase K Chain: A
Molecule details ›
Chain: A
Length: 279 amino acids
Theoretical weight: 28.93 KDa
Source organism: Parengyodontium album
UniProt:
  • Canonical: P06873 (Residues: 106-384; Coverage: 76%)
Gene name: PROK
Sequence domains: Subtilase family
Structure domains: Peptidase S8/S53 domain

Ligands and Environments

1 bound ligand:
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 1.6Å
Relevant EMDB volumes: EMD-8221

Quick links

Citations

23 review citations

MicroED in natural product and small molecule research.
Danelius et al. (2021)
22 more

6 mentions without citation

MicroED opens a new era for biological structure determination.
Nannenga et al. (2016)
5 more