› 5k9s

X-ray diffraction
2.4Å resolution

Importin-alpha in complex with HNF1-beta peptide

Released:
DOI: 10.2210/pdb5k9s/pdb
PDB entry 5k9s coloured by chain, front view.
PDB entry 5k9s coloured by chain, side view.
PDB entry 5k9s coloured by chain, top view.
Source organisms:
Entry authors: Wiedmann MM, Aibara S, Spring DR, Stewart M, Brenton J

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152996 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Importin subunit alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 458 amino acids
Theoretical weight: 49.64 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P52293 (Residues: 72-529; Coverage: 87%)
Gene names: Kpna2, Rch1
Sequence domains:
Structure domains: Leucine-rich Repeat Variant
Hepatocyte nuclear factor 1-beta Chains: B, C
Molecule details ›
Chains: B, C
Length: 11 amino acids
Theoretical weight: 1.48 KDa
Source organism: Homo sapiens
Expression system: synthetic construct
UniProt:
  • Canonical: P35680 (Residues: 227-237; Coverage: 2%)
Gene names: HNF1B, TCF2

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: C2221
Unit cell:
a: 81.38Å b: 111.394Å c: 129.358Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.185 0.221
Expression systems:
  • Escherichia coli BL21(DE3)
  • synthetic construct

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