› 5kal

X-ray diffraction
2.75Å resolution

Terminal uridylyl transferase 4 from Trypanosoma brucei with bound UTP and UpU

Released:
DOI: 10.2210/pdb5kal/pdb
PDB entry 5kal coloured by chain, front view.
PDB entry 5kal coloured by chain, side view.
PDB entry 5kal coloured by chain, top view.
Source organism: Trypanosoma brucei
Primary publication:
RNA Editing TUTase 1: structural foundation of substrate recognition, complex interactions and drug targeting.
Rajappa-Titu L, Suematsu T, Munoz-Tello P, Long M, Demir Ö, Cheng KJ, Stagno JR, Luecke H, Amaro RE, Aphasizheva I, Aphasizhev R, Thore S
Nucleic Acids Res 44 10862-10878 (2016)
PMID: 27744351

Function and Biology Details

Reaction catalysed: 
UTP + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174325 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Terminal uridylyltransferase 4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 353 amino acids
Theoretical weight: 39.88 KDa
Source organism: Trypanosoma brucei
Expression system: Escherichia coli
UniProt:
  • Canonical: Q381M1 (Residues: 1-333; Coverage: 100%)
Gene names: TUT4, Tb11.01.7300
Sequence domains: Cid1 family poly A polymerase
RNA (5'-R(*UP*U)-3') Chains: Y, Z
Molecule details ›
Chains: Y, Z
Length: 2 nucleotides
Theoretical weight: 567 Da
Source organism: Trypanosoma brucei
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand UTP 2 x UTP
Ligand MG 5 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: P21
Unit cell:
a: 82.688Å b: 42.876Å c: 109.618Å
α: 90° β: 94.05° γ: 90°
R-values:
R R work R free
0.216 0.214 0.259
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

2 review citations

Lexis and Grammar of Mitochondrial RNA Processing in Trypanosomes.
Aphasizheva et al. (2020)
1 more

3 mentions without citation

Multi-domain utilization by TUT4 and TUT7 in control of let-7 biogenesis.
Faehnle et al. (2017)
2 more